Possibility to Improve the Cyanobacterial Poly-β-Hydroxybutyrate Biosynthesis Level

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

GUIFANG WU²  QINGYU WU¹  ZHONGYAO SHEN² 

[1] Department of Biological Sciences and Biotechnology, Tsinghua University;Department of Chemical Engineering, Biochemical Engineering Institute, Tsinghua University

关键词: Biodegradable Plastic;    Cyanobacterium;    Poly-β-hydroxybutyrate;    Two-Step Culture;    Nitrogen-Starvation;   

DOI  :  10.1252/jcej.34.1187

来源: Maruzen Company Ltd

【 摘 要 】

References(7)The biodegradable plastic poly-β-hydroxybutyrate (PHB) was synthesized by a unicellular cyanobacterium, Synechocystis sp. PCC6803. The cell growth and PHB accumulation were inspected comprehensively. Nitrogen starvation was favorable for cyanobacterial PHB accumulation, while the final biomass of nitrogen-starved culture decreased to one-third of that in nitrogen-sufficient condition. A two-step culture, a balanced mixotrophic culture followed by a nitrogen-starved culture, was developed to improve the cyanobacterial PHB production efficiency. To adapt the large-scale outdoor culture that has the natural light/dark cycle, modification to the culture method was put forward based on the studies on the heterotrophic culture.

【 授权许可】

Unknown