Protein PII regulates both inorganic carbon and nitrate uptake and is modified by a redox signal in Synechocystis PCC 6803

FEBS Letters

Tandeau de Marsac, Nicole²  Joset, Françoise¹  Bédu, Sylvie¹  Jeanjean, Robert¹  Hisbergues, Michael¹ 

[1] Laboratoire de Chimie Bactérienne, CNRS, 31 Chemin J. Aiguier, 13402 Marseille Cedex 20, France;Unité de Physiologie Microbienne, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France

关键词: Protein PII;    Cyanobacterium;    Nitrate uptake;    Bicarbonate uptake;    Phosphorylation;    Redox state;   

DOI  :  10.1016/S0014-5793(99)01624-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Synechocystis PCC 6803 as in other cyanobacteria, involvement of protein PII in the co-regulation of inorganic carbon and nitrogen metabolism was established based on post-translational modifications of the protein resulting from changes in the carbon/nitrogen regimes. Uptake of bicarbonate and nitrate in response to changes of the carbon and/or nitrogen regimes is altered in a PII-null mutant, indicating that both processes are under control of PII. Modulation of electron flow by addition of methyl viologen with or without duroquinol, or in a NAD(P)H dehydrogenase-deficient mutant, affects the phosphorylation level of PII. The redox state of the cells would thus act as a trigger for PII phosphorylation.

【 授权许可】

Unknown