CONTINUOUS SEPARATION OF PROTEIN FROM COLLOIDAL SOLUTION CONTAINING SEVERAL KINDS OF PROTEINS WITH ISOELECTRIC FOCUSING

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

TERUHIKO HOSHINO¹  HIROSHI YUKAWA¹  TOSHIYUKI KASAKOSHI¹  MASARU HAKODA¹ 

[1] Department of Chemical Engineering, Gunma University

关键词: Isoelectric Focusing;    Electrophoresis;    Carrier Ampholyte;    Protein;    Continuous Separation;   

DOI  :  10.1252/jcej.21.262

来源: Maruzen Company Ltd

【 摘 要 】

References(6)Cited-By(1)The continuous separation of each protein from a colloidal solution containing several kinds of proteins was investigated by isoelectric focusing. A separation device comprising three separation cells divided by semipermeable Teflon membranes and two electrode compartments was used in this experiment. A colloidal solution containing bovine serum albumin and bovine hemoglobin was used as a sample and ampholytes were used as a buffer reagent. The costly ampholytes were recovered from effluent by ultrafiltration for reuse as buffer solution. The equation for separation efficiency was analysed semi-theoretically and the adaptability of the equation was examined experimentally. A separation efficiency of over 95 % was obtained.

【 授权许可】

Unknown