Diagnostic role and estimation of Adenosine Deaminase (ADA) in serosal effusions

International Journal of Biomedical and Advance Research

Akash Gupta¹  Shailja Sharma¹  Meenakshi Panthari¹  Naved Ahmad¹ 

关键词: ADA;    Pleural effusion;    Peritoneal effusion;    Synovial effusion;    TB;   

DOI  :  10.7439/ijbar.v4i5.346

学科分类：药学、药理学、毒理学（综合）

来源: Scholar Science Journals

【 摘 要 】

Objective?The objective of the study was to estimate the minimum value of adenosine deaminase (ADA) in tubercular (TB) and non tubercular serosal effusions. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? Methods?The study was conducted on 336 subjects attending to OPD & IPD of TB chest & respiratory disease, Medicine and Orthopedics departments of Subharti Medical College and its associated hospital, Meerut, U.P. India. Out of these 155 subjects were tubercular (tubercular pleural effusion 45, peritoneal 34, synovial 34 and cerebrospinal fluid 42) and 181 were non tubercular (pleural 42, peritoneal 48, synovial 37 and cerebrospinal fluid 54). ADA levels of different serosal fluids were estimated by Microexpress kit, based on Guisti & Galanti method. Data was analyzed by one way Anova.??????????????? ???????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????????Results?In our study, we found the following cut off values of ADA in different types of serosal effusions. In tubercular pleural effusion it was 65Unit/Litre (U/L), peritoneal 75U/L, synovial 42U/L and cerebrospinal fluid 13U/L respectively. In non tubercular pleural effusion it was 6U/L, peritoneal 4U/L, synovial 13U/L and cerebrospinal fluid 2U/L respectively. Sensitivity & specificity of tubercular effusion were 100% and 0% respectively. While for non tubercular pleural effusion it was 93% & 53%, for peritoneal 90% & 67%, for synovial & CSF it was 0% & 100% respectively. ????????????????????????????????????????????????????????????????????????????????????????????????????????????????????? Conclusion ? ADA estimation will be helpful as an alternative diagnostic method for early detection of TB and to differentiate between pulmonary and extra pulmonary tuberculosis.?

【 授权许可】

CC BY