Anti-inflammatory effect of apitoxin and Apis mellifera on prostaglandin E2 in gingival crevicular fluid of patients with and without periodontal disease, submitted to apitherapy: preliminary test

Revista clínica de periodoncia, implantología y rehabilitación oral

Faúndez Poblete, W¹  Burgos Arias, A¹  Universidad del Desarrollo, Concepción, Chile¹  Narváez Carrasco, CG¹ 

关键词: Periodontal disease;    simple radial immunodiffusion;    apitherapy;    apitoxin;    gingival crevicular fluid;    prostaglandin E2.;   

DOI  :  10.4067/S0719-01072011000200005

来源: Sociedad de Periodoncia de Chile

【 摘 要 】

Introduction: Apitoxin, which is produced by Apis mellifera, has anti-inflammatory effect on a number of biomarkers. Prostaglandin E2 is one of them, being present in gingival crevicular fluid (GCF). Prostaglandin E2 is evidenced in periodontal disease. Objective: This study evaluated the antiinflammatory effect of apitoxin on concentration of prostaglandin E2 FGC in a patient with no periodontal disease (SEP) and other with periodontal disease (CEP). Materials and Methods: We selected both a SEP and CEP patient who were subjected to apitherapy for 28 days. There were 5 samples per patient of FGC, being stored, centrifuged and refrigerated for their preservation. Subsequently, the concentrations of crevicular prostaglandin E2 were measured by simple radial immunodiffusion in petri dishes with antibody concentration of prostaglandin E2 of 1:1000. Sealed at 4 °C, after 72 hours to allow diffusion, they were stained with Coomassie Brilliant Blue, determining the concentration of each plate. Results: SEP patient immediately before apitherapy presented a concentration of 0.9636 ± 0.0055 (g / mL), ending with a concentration of 0.9196 ± 0.0733 (g / mL) upon completion of 28 days of treatment. CEP patient before receiving apitherapy showed a concentration of 1.1866 ± 0.0867 (g/mL), ending with a concentration of 0.9858 ± 0.0074 (g/mL) upon completion of 28 days of treatment. Discussion: The findings of this study show a decrease in the concentration of PGE2 of FGC both for the CEP and SEP patient subjected to apitherapy for 28 days, being this decrease 3.7 times higher in the CEP patient.

【 授权许可】

Unknown