Antimicrobial activity of apitoxin, melittin and phospholipase A2 of honey bee (Apis mellifera) venom against oral pathogens

Anais da Academia Brasileira de Ciências

Luís F. Leandro¹  Carlos A. Mendes¹  Luciana A. Casemiro¹  Adriana H.c. Vinholis¹  Wilson R. Cunha¹  Rosana De Almeida¹  Carlos H.g. Martins¹ 

关键词: antibacterial activity;    apitoxin;    Melittin;    Phospholipase A2;    tooth decay;    oral pathogens;    atividade antibacteriana;    apitoxina;    Melitina;    Fosfolipase A2;    cárie dental;    patógenos bucais;   

DOI  :  10.1590/0001-3765201520130511

来源: SciELO

【 摘 要 】

In this work, we used the Minimum Inhibitory Concentration (MIC) technique to evaluate the antibacterial potential of the apitoxin produced by Apis mellifera bees against the causative agents of tooth decay. Apitoxin was assayed in naturaand in the commercially available form. The antibacterial actions of the main components of this apitoxin, phospholipase A2, and melittin were also assessed, alone and in combination. The following bacteria were tested: Streptococcus salivarius, S. sobrinus, S. mutans, S. mitis, S. sanguinis, Lactobacillus casei, and Enterococcus faecalis. The MIC results obtained for the commercially available apitoxin and for the apitoxin in natura were close and lay between 20 and 40µg / mL, which indicated good antibacterial activity. Melittin was the most active component in apitoxin; it displayed very promising MIC values, from 4 to 40µg / mL. Phospholipase A2 presented MIC values higher than 400µg / mL. Association of mellitin with phospholipase A2 yielded MIC values ranging between 6 and 80µg / mL. Considering that tooth decay affects people's health, apitoxin and its component melittin have potential application against oral pathogens.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License