Enzymatic synthesis isoamyl butyrate employing commercial microbial lipases

Química Nova

Universidade Federal do Rio Grande, Rio Grande, Brasil¹  Burkert, Carlos André Veiga¹  Kalil, Susana Juliano¹  Aragão, Vitor Cardoso¹  Thiesen, Cleidi¹  Burkert, Janaína Fernandes de Medeiros¹  Porciuncula, Barbara Daniele Almeida¹  Anschau, Andréia¹ 

关键词: enzymatic esterification;    organic media;    lipase.;   

DOI  :  10.1590/S0100-40422009000900005

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Isoamyl butyrate production was investigated using free and immobilized lipases by esterification of butyric acid with isoamyl alcohol in a solvent-free system and in an organic media. Among the enzymes studied, Lipozyme TL IM was found to be the most active catalyst in n-hexane as a solvent. The effects of different solvents and the amount of water added on conversion rates were studied. A maximum conversion yield of 80% in n-hexano at 48 h was obtained under the following conditions: 3 g L-1 of Lipozyme TL IM, 30 ºC, 180 rpm of agitation, isoamyl alcohol to butyric acid molar ratio of 1:1 and acid substrate concentration of 0.06 M.

【 授权许可】

Unknown