Enzymatic synthesis isoamyl butyrate employing commercial microbial lipases

Quimica nova

Kalil, Susana Juliano¹  Burkert, Carlos André Veiga¹  Aragão, Vitor Cardoso¹  Thiesen, Cleidi¹  Burkert, Janaína Fernandes de Medeiros¹  Porciuncula, Barbara Daniele Almeida¹  Anschau, Andréia¹ 

[1] Universidade Federal do Rio Grande, Rio Grande, Brasil

关键词: enzymatic esterification;    organic media;    lipase.;   

DOI  :  10.1590/S0100-40422009000900005

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Isoamyl butyrateproduction was investigated using free and immobilized lipases by esterificationof butyric acid with isoamyl alcohol in a solvent-free system and in an organicmedia. Among the enzymes studied, Lipozyme TL IM was found to be the most activecatalyst in n-hexane as a solvent. The effects of different solvents and theamount of water added on conversion rates were studied. A maximum conversionyield of 80% in n-hexano at 48 h was obtained under the following conditions:3 g L-1 of Lipozyme TL IM, 30 ºC, 180 rpm of agitation, isoamyl alcoholto butyric acid molar ratio of 1:1 and acid substrate concentration of 0.06M.

【 授权许可】

CC BY