Síntese enzimática de butirato de isoamila empregando lipases microbianas comerciais

Química Nova

Vitor Cardoso Aragão¹  Andréia Anschau¹  Barbara Daniele Almeida Porciuncula¹  Cleidi Thiesen¹  Susana Juliano Kalil¹  Carlos André Veiga Burkert¹  Janaína Fernandes De Medeiros Burkert¹ 

[1] ,Universidade Federal do Rio Grande Escola de Química e Alimentos Rio Grande RS ,Brasil

关键词: enzymatic esterification;    organic media;    lipase;   

DOI  :  10.1590/S0100-40422009000900005

来源: SciELO

【 摘 要 】

Isoamyl butyrate production was investigated using free and immobilized lipases by esterification of butyric acid with isoamyl alcohol in a solvent-free system and in an organic media. Among the enzymes studied, Lipozyme TL IM was found to be the most active catalyst in n-hexane as a solvent. The effects of different solvents and the amount of water added on conversion rates were studied. A maximum conversion yield of 80% in n-hexano at 48 h was obtained under the following conditions: 3 g L-1 of Lipozyme TL IM, 30 ºC, 180 rpm of agitation, isoamyl alcohol to butyric acid molar ratio of 1:1 and acid substrate concentration of 0.06 M.

【 授权许可】

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