Validation of a high performace liquid chromatography method for the determina of lamivudine and zidovudine in tablets

Química Nova

Beck, Ruy Carlos Ruver¹  Oliveira, Fernanda Kreutz de¹  Dalmora, Sérgio Luiz¹  Universidade Federal de Santa Maria, Santa Maria, Brasil¹  Cardoso, Simone Gonçalves¹  Athayd, Margareth Linde¹  Codevilla, Cristiane¹ 

关键词: HPLC;    lamivudine;    zidovudine.;   

DOI  :  10.1590/S0100-40422007000500033

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

An HPLC method was validated to assay lamivudine and zidovudine combined in tablets. The chromatographic separation was carried out using methanol and acetate buffer pH 6.5 (50:50 v/v) and a RP-18 column, as mobile and stationary phase, respectively. The UV detection was at 270 nm. The method was linear in the range of 24 – 36 µg/mL (lamivudine) and 48 – 72 µg/mL (zidovudine). The recovery (accuracy) ranged from 101.35% to 103.04% and the precision (repeatability and intermediate precision) was less than 2%. The method can be also applied to the quantification of these drugs in the dissolution test of tablets containing both drugs.

【 授权许可】

Unknown