Atomic force microscopy applied to immunoassays

Química Nova

Yamanaka, Hideko¹  Ferreira, Antonio Aparecido Pupim¹  Universidade Estadual Paulista, Araraquara¹ 

关键词: atomic force microscopy;    antigen-antibody;    immunoassay.;   

DOI  :  10.1590/S0100-40422006000100024

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Affinity reactions have been used for specific detection of their complementary partners and an enormous variety of enzyme-linked immunosorbent assay (ELISA) formats are used in research and in routine serological tests. With the advent of the atomic force microscopy (AFM) technique, the immune reactions have been monitored by these devices. In the present article we focus on applications of AFM to immunoassays. After introducing the basic concepts of AFM, a brief discussion on the monitoring of the interactions between antigens and antibodies through both topographic image and biosensor systems is presented.

【 授权许可】

Unknown