Microscopia de força atômica aplicada em imunoensaios

Química Nova

Antonio Aparecido Pupim Ferreira²  Hideko Yamanaka¹ 

[1] ,Universidade Estadual Paulista Instituto de Química Departamento de Bioquímica e Tecnologia QuímicaAraraquara SP

关键词: atomic force microscopy;    antigen-antibody;    immunoassay;   

DOI  :  10.1590/S0100-40422006000100024

来源: SciELO

【 摘 要 】

Affinity reactions have been used for specific detection of their complementary partners and an enormous variety of enzyme-linked immunosorbent assay (ELISA) formats are used in research and in routine serological tests. With the advent of the atomic force microscopy (AFM) technique, the immune reactions have been monitored by these devices. In the present article we focus on applications of AFM to immunoassays. After introducing the basic concepts of AFM, a brief discussion on the monitoring of the interactions between antigens and antibodies through both topographic image and biosensor systems is presented.

【 授权许可】

CC BY-NC   
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