Atomic force microscopy applied to immunoassays

Quimica nova

Yamanaka, Hideko¹  Ferreira, Antonio Aparecido Pupim¹ 

[1] Universidade Estadual Paulista, Araraquara

关键词: atomic force microscopy;    antigen-antibody;    immunoassay.;   

DOI  :  10.1590/S0100-40422006000100024

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Affinity reactionshave been used for specific detection of their complementary partners and anenormous variety of enzyme-linked immunosorbent assay (ELISA) formats are usedin research and in routine serological tests. With the advent of the atomicforce microscopy (AFM) technique, the immune reactions have been monitored bythese devices. In the present article we focus on applications of AFM to immunoassays.After introducing the basic concepts of AFM, a brief discussion on the monitoringof the interactions between antigens and antibodies through both topographicimage and biosensor systems is presented.

【 授权许可】

CC BY