An HPLC-DAD method to quantification of main phenolic compounds from leaves of Cecropia Species

Journal of the Brazilian Chemical Society

Schenkel, Eloir P.¹  Ortmann, Caroline F.¹  Costa, Geison M.¹  Universidade Federal de Santa Catarina, Florianópolis, Brazil¹  Reginatto, Flávio H.¹ 

关键词: Cecropia glaziovii;    Cecropia pachystachya;    HPLC-DAD;    C-glycosylflavonoids;    chlorogenic acid;   

DOI  :  10.1590/S0103-50532011000600014

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

An efficient and reproducible HPLC-DAD method was developed and validated for the simultaneous quantification of major compounds (chlorogenic acid, isoorientin, orientin and isovitexin) present in the leaves of two Cecropia species, C. glaziovii and C. pachystachya. From the leaves of C. glaziovii and C. pachystachya were isolated the C-glycosylflavones isoorientin and isovitexin and identified on both species chlorogenic acid (3-O-caffeoylquinic acid) and the O-glycosylflavonol isoquercitrin. The C-glycosylflavone orientin was isolated only from C. pachystachya. Chlorogenic acid was the major compound in both species (11.1 mg g-1 of extract of C. glaziovii and 27.2 mg g-1 of extract of C. pachystachya) and for the flavonoids quantified, isovitexin was the main C-glycosylflavonoid for C. glaziovii (4.6 mg g-1 of extract) and isoorientin the main one for C. pachystachya (17.3 mg g-1 of extract).

【 授权许可】

Unknown