Visible LED-Based Instrumentation for Photometric Determination of Electroosmotic Flow in Microchannels

Journal of the Brazilian Chemical Society

Silva, José Alberto Fracassi da¹  Universidade Estadual de Campinas, Campinas, Brazil¹  Coltro, Wendell Karlos T.¹  Deblire, Ariane¹  Jesus, Dosil P. de¹  Universidade Federal de Goiás, Goiânia, Brazil¹  Instituto Nacional de Ciência e Tecnologia em Bioanalítica, Campinas, Brazil¹ 

关键词: electroosmotic flow;    μTAS;    Lab-on-a-chip;    photometric methods;    microfluidic devices;   

DOI  :  10.1590/S0103-50532011000400017

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

An instrumental setup for electroosmotic flow (EOF) determination in microfluidic devices is described. The system is based on a trinocular microscope and an integrated photodiode OPT101. The radiation was provided by a high-intensity white LED. For the determinations, patent blue V and methylene blue aqueous solutions were used. The setup was applied for EOF monitoring in hybrid toner/glass and PDMS microchips. For the toner/glass device, the EOF mobility was determined to be (5.75 ± 0.01) 10-4 cm2 V-1 s-1 at pH 7.0. For PDMS devices the measured EOF mobility was (3.2 ± 0.1) 10-4 cm2 V-1  s-1. Reproducible measurements were obtained in all experiments, which produced results with small errors. The proposed method was compared to the conventional current monitoring method.

【 授权许可】

Unknown