Proposal of Pumpless, Valveless, and Flowless Miniaturized Reactor Using Magnetic Beads for the Portable Analysis Device

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

Kentaro Takayanagi¹  Mina Okochi³  Hiroshi Ito³  Kazuo Sato¹  Mitsuhiro Shikida²  Hiroyuki Honda³ 

[1] Department of Micro-Nano System Engineering, Graduate School of Engineering, Nagoya University;EcoTopia Science Institute, Nagoya University;Department of Biotechnology, Graduate School of Engineering, Nagoya University

关键词: Bioreactor;    Magnetic Beads;    Droplet Handling;    Portable Analysis Device;    μTAS;   

DOI  :  10.1252/jcej.39.1296

来源: Maruzen Company Ltd

【 摘 要 】

References(5)Cited-By(4)A miniaturized chemical-analysis system based on magnetic beads-droplet-handling mechanism without pumps and valves was developed for analysis. The analysis system has possibility for the development of a portable analysis device since there is no complicated mechanical component. In this report, we constructed an analysis system with 8 wells connected by channels. Magnetic beads with a diameter of 22.0 μm were used as a mobile phase. We demonstrated the detection of biotin-labeled DNA oligomers using this system. The result showed that this detection system has specificity and dependence of the signal on the target DNA concentration.

【 授权许可】

Unknown