AGRIVITA, Journal of Agricultural Science | |
ISOLATION AND CHARACTERIZATION OF A MOLYBDENUM-REDUCING AND GLYPHOSATE-DEGRADING Klebsiella oxytoca STRAIN SAW-5 IN SOILS FROM SARAWAK | |
M.K. Sabullah3  S.A. Ahmad4  N.A. Shamaan2  M.F. Rahman4  M.S. Shukor1  M.Y. Shukor4  M.R. Sulaiman5  | |
[1] Snoc International Sdn Bhd, Lot 343, Jalan 7/16 Kawasan Perindustrian Nilai 7, Inland Port, 71800, Negeri Sembilan, Malaysia$$;Faculty of Medicine and Health Sciences, Islamic Science University of Malaysia,55100 Kuala Lumpur, Malaysia$$;Faculty of Science and Natural Resources, Universiti Malaysia Sabah,88400 Kota Kinabalu, Sabah, Malaysia$$;Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia,UPM 43400 Serdang, Selangor, Malaysia$$;School of Food Science and Nutrition, Universiti Malaysia Sabah, Malaysia$$ | |
关键词: bioreduction; glyphosate; Klebsiella oxytoca; molybdenum blue; molybdenum-reducing bacterium; | |
DOI : 10.17503/agrivita.v38i1.654 | |
学科分类:农业科学(综合) | |
来源: Universitas Brawijaya * Fakultas Pertanian / Brawijaya University, Faculty of Agriculture | |
【 摘 要 】
Bioremediation of pollutants including heavy metals and xenobiotics is an economic and environmentally friendly process. A novel molyb-denum-reducing bacterium with the ability to utilize the pesticide glyphosate as a carbon source is reported. The characterization works were carried out utilizing bacterial resting cells in a microplate format. The bacterium reduces molybdate to Mo-blue optimally between pH 6.3 and 6.8 and at 34 o C. Glucose was the best elec-tron donor for supporting molybdate reduction followed by lactose, maltose, melibiose, raffinose, d-mannitol, d-xylose, l-rhamnose, l-arabinose, dulcitol, myo-inositol and glycerol in descending order. Other requirements include a phosphate concentration at 5.0 mM and a molybdate concentration between 20 and 30 mM. The molybdenum blue exhibited an absorption spec-trum resembling a reduced phospho-molybdate. Molybdenum reduction was inhibited by mercury, silver, cadmium and copper at 2 ppm by 45.5, 26.0, 18.5 and 16.3%, respectively. Biochemical analysis identified the bacterium as Klebsiella oxytoca strain Saw-5. To conclude, the capacity of this bacterium to reduce molybdenum into a less toxic form and to grow on glyphosate is novel and makes the bacterium an important instrument for bioremediation of these pollutants.
【 授权许可】
Unknown
【 预 览 】
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