The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells

Cellular & Molecular Biology Letters

Delphine T. Marie-Egyptienne²  Chantal Autexier⁵  Johans Fakhoury³  Laetitia Trapp-Fragnet⁴  Denis Rasschaert¹ 

[1] UFR Sciences et Techniques, Université François Rabelais de Tours, Tours, France$$;Lady Davis Institute for Medical Research, Jewish General Hospital Montréal, Montréal, Canada$$Department of Anatomy and Cell Biology, McGill University, Montréal, Canada$$Ontario Cancer Institute, Princess Margaret Hospital, Ontario, Canada$$;Lady Davis Institute for Medical Research, Jewish General Hospital Montréal, Montréal, Canada$$Department of Medicine, Experimental Medicine Division, McGill University, Montréal, Canada$$Department of Chemistry, McGill University, Montreal, Canada$$;INRA, UR1282 Infectiologie et Santé Publique, Nouzilly, France$$;Lady Davis Institute for Medical Research, Jewish General Hospital Montréal, Montréal, Canada$$Department of Anatomy and Cell Biology, McGill University, Montréal, Canada$$Department of Medicine, Experimental Medicine Division, McGill University, Montréal, Canada$$

关键词: Telomerase;    Holoenzyme;    hTERT;    vTR;    Dyskerin;    Marek’s disease virus;   

DOI  :  10.2478/s11658-012-0031-6

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

The minimal vertebrate telomerase enzyme is composed of a protein component (telomerase reverse transcriptase, TERT) and an RNA component (telomerase RNA, TR). Expression of these two subunits is sufficient to reconstitute telomerase activity in vitro, while the formation of a holoenzyme comprising telomerase-associated proteins is necessary for proper telomere length maintenance. Previous reports demonstrated the high processivity of the human telomerase complex and the interspecies compatibility of human TERT (hTERT). In this study, we tested the function of the only known viral telomerase RNA subunit (vTR) in association with human telomerase, both in a cell-free system and in human cells. When vTR is assembled with hTERT in a cell-free environment, it is able to interact with hTERT and to reconstitute telomerase activity. However, in human cells, vTR does not reconstitute telomerase activity and could not be detected in the human telomerase complex, suggesting that vTR is not able to interact properly with the proteins constituting the human telomerase holoenzyme.

【 授权许可】

Unknown