Expression of telomerase inhibits hydroxyl radical‐induced apoptosis in normal telomerase negative human lung fibroblasts

FEBS Letters

Ren, Jian-Guo¹  Cai, Guo-Ping¹  Xia, Hui-Li¹  Dai, Yao-Ren¹  Tian, Yan-Mei¹  Just, Tom² 

[1] Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, PR China;Department of Probe Applications, DAKO A/S, Produktionsvej 42, DK-2600 Glostrup, Denmark

关键词: Telomerase;    Telomere;    Apoptosis;    Hydroxyl radical;    Human telomerase reverse transcriptase subunit;    hTERT;    human telomerase reverse transcriptase;    hLF;    human lung fibroblast;    OH;    hydroxyl radical;    DAPI;    4′;    6-diamidino-2-phenylindole;    TUNEL;    terminal deoxynucleotidyltransferase (TdT)-mediated dUTP-digoxigenin nick end labeling;    PI;    propidium iodide;    TRAP;    telomeric repeat amplification protocol;   

DOI  :  10.1016/S0014-5793(00)02397-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In tumor cells telomerase activity is associated with resistance to apoptosis and the introduction of the human telomerase reverse transcriptase (hTERT) subunit into normal human cells is associated with life span extension of the cells. To determine the role of telomerase in regulating apoptosis, telomerase negative human embryo lung fibroblasts were transfected with the hTERT gene. Unlike the control fibroblasts, the telomerase-expressing cells had elongated telomeres and were resistant to apoptosis induced by hydroxyl radicals. The results indicate that expression of telomerase and, thus, the maintenance of telomere length in normal human somatic cells caused resistance to not only cellular senescence but also apoptosis. Moreover, we found that hydroxyl radical-induced apoptosis in telomerase-expressing and control fibroblasts was caspase-3 independent. These findings have revealed a new type of interrelation between telomerase and caspase-3, which may indicate that in this case the expressed telomerase may inhibit apoptosis at a site not related to the caspase-3 cascade.

【 授权许可】

Unknown