Human adipose-derived stem cells for the treatment of intracerebral hemorrhage in rats via femoral intravenous injection

Cellular & Molecular Biology Letters

Ting-Yi Lee¹  Hock-Kean Liew⁵  Shin-Yuan Chen⁴  Cheng-Yoong Pang⁶  Tzu-Yung Chen²  Kuo-Liang Yang⁷  Py-Yu Lin¹  Shee-Ping Chen¹  Jiunn-Tat Lee³ 

[1] Buddhist Tzu Chi Stem Cells Center, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan$$;Department of Neurosurgery, Buddhist Tzu Chi Medical Center Taichung Branch, Buddhist Compassion Relief Tzu Chi Foundation, Taichung, Taiwan$$;Division of Plastic Surgery, Department of Surgery, Buddhist Tzu Chi Medical Center and Buddhist Tzu Chi University, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan$$;Division of Functional Neuroscience, Department of Neurosurgery, Neuro-Medical Scientific Center, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan$$;Department of Research, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan$$;Department of Research, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan$$Graduate Institute of Clinical Medicine, Buddhist Tzu Chi University, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan$$;Buddhist Tzu Chi Stem Cells Center, Buddhist Tzu Chi Medical Center, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan$$Institute of Medical Biotechnology, Buddhist Tzu Chi University, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan$$

关键词: Adipose-derived stem cells;    Adult stem cells;    Adipose tissue;    Intracerebral hemorrhage;    Intravenous stem cell injection;    Neural stem cells;    Regenerative medicine;    Stem cell therapy;    Stroke;    Stromal cells;   

DOI  :  10.2478/s11658-012-0016-5

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Human adipose-derived stem cells (huADSC) were generated from fat tissue of a 65-year-old male donor. Flow cytometry and reverse transcription polymerase chain reaction (RT-PCR) analyses indicated that the huADSC express neural cell proteins (MAP2, GFAP, nestin and β-III tubulin), neurotrophic growth factors (BDNF and GDNF), and the chemotactic factor CXCR4 and its corresponding ligand CXCL12. In addition, huADSC expressed the characteristic mesenchymal stem cell (MSC) markers CD29, CD44, CD73, CD90, CD105 and HLA class I. The huADSC were employed, via a right femoral vein injection, to treat rats inflicted with experimental intracerebral hemorrhage (ICH). Behavioral measurement on the experimental animals, seven days after the huADSC therapy, showed a significant functional improvement in the rats with stem cell therapy in comparison with rats of the control group without the stem cell therapy. The injected huADSC were detectable in the brains of the huADSC treated rats as determined by histochemistry analysis, suggesting a role of the infused huADSC in facilitating functional recovery of the experimental animals with ICH induced stroke.

【 授权许可】

Unknown