Acheron, an novel LA antigen family member, binds to cask and forms a complex with id transcription factors

Cellular & Molecular Biology Letters

Chul Kim³  Christos Valavanis⁴  Lawrence M. Schwartz¹  Haifeng Weng²  Zhaohui Wang³ 

[1] Molecular and Cellular Biology Program, Morrill Science Center, University of Massachusetts, Amherst, USA$$Department of Biology, Morrill Science Center, University of Massachusetts, Amherst, USA$$Pioneer Valley Life Sciences Institute, Springfield, USA$$;Molecular and Cellular Biology Program, Morrill Science Center, University of Massachusetts, Amherst, USA$$Department of Biology, Northeastern University, Boston, USA$$;Molecular and Cellular Biology Program, Morrill Science Center, University of Massachusetts, Amherst, USA$$;Department of Biology, Morrill Science Center, University of Massachusetts, Amherst, USA$$Molecular Pathology and Genetics Division, Department of Pathology, Metaxa Cancer Hospital, Piraeus, Greece$$

关键词: Programmed cell death;    Muscle;    Apoptosis;    Lupus antigen protein;   

DOI  :  10.2478/s11658-008-0046-1

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Acheron, a Lupus antigen ortholog, was identified as a novel death-associated transcript from the intersegmental muscles of the mothManduca sexta. Acheron is phylogenetically-conserved and represents a new sub-family of Lupus antigen proteins. Acheron is expressed predominantly in neurons and muscle in vertebrates, and regulates several developmental events including myogenesis, neurogenesis and possibly metastasis. Using Acheron as bait, we performed a yeast two-hybrid screen with a mouse embryo cDNA library and identified CASK-C, a novel CASK/Lin-2 isoform, as an Acheron binding partner. Acheron and CASK-C bind via the C-terminus of Acheron and the CaMKII-like domain of CASK-C. Co-immunoprecipitation assays verify this interaction and demonstrate that Acheron also forms a complex with all members of the Id (inhibitor of differentiation) proteins. Taken together, these data suggest a mechanism by which Acheron may regulate development and pathology.

【 授权许可】

Unknown