The inhibition of in vivo tumorigenesis of osteosarcoma (OS)-732 cells by antisense human osteopontin RNA

Cellular & Molecular Biology Letters

Yan Xue³  Li-Qiu Sun²  Lin Zhang⁴  Xiu-Mei Sui⁶  Dao-Qiang Zhang⁶  Ya-Jun Liu³  Si-Jin Liu¹  Guo-Fa Hu⁵  Zi-Wei Cai² 

[1] Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China$$Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, USA$$;Department of Pathophysiology, Medical College of Shantou University, Shantou, China$$;Beijing Jishuitan Hospital, Peking University, Beijing, China$$;Research Institute of Sports Medicine, Soochow University, Suzhou, China$$;Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China$$;Wendeng Central Hospital, Weifang Medical College, Weihai, China$$

关键词: Osteopontin;    Osteosarcoma;    Antisense RNA;   

DOI  :  10.2478/s11658-007-0031-0

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Osteopontin (OPN) is a secreted, non-collagenous, sialic acid-rich protein which functions by mediating cell-matrix interactions and cellular signaling via binding with integrins and CD44 receptors. An increasing number of studies have shown that OPN plays an important role in controlling cancer progression and metastasis. OPN was found to be expressed in many human cancer types, and in some cases, its over-expression was shown to be directly associated with poor patient prognosis. In vitro cancer cell line and animal model studies have clearly indicated that OPN can function in regulating the cell signaling that ultimately controls the oncogenic potential of various cancers. Previous studies in our laboratory demonstrated that OPN is highly expressed in human osteosarcoma (OS) cell line OS-732. In this study, we successfully reduced the tumorigenecity of OS-732 cells xenotransplanted into nude mice, using the antisense human OPN (hOPN) RNA expression vector.

【 授权许可】

Unknown