Application of aptamers in diagnostics, drug-delivery and imaging

Journal of biosciences

MARCO G CASTELEIJN²  ARTO URTTI²  MUNIASAMY NEERATHILINGAM1¹  CHETAN CHANDOLA¹  SHEETAL KALME¹ 

[1] Center for Cellular and Molecular Platforms, NCBS-TIFR, Bangalore 560 065, India$$;Centre for Drug Research, Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland$$

关键词: Aptamer;    cancer;    conjugation-methods;    diagnostics;    drug-delivery;    imaging;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Aptamers are small, single-stranded oligonucleotides (DNA or RNA) that bind to their target with high specificity andaffinity. Although aptamers are analogous to antibodies for a wide range of target recognition and variety ofapplications, they have significant advantages over antibodies. Since aptamers have recently emerged as a class ofbiomolecules with an application in a wide array of fields, we need to summarize the latest developments herein. Inthis review we will discuss about the latest developments in using aptamers in diagnostics, drug delivery and imaging.We begin with diagnostics, discussing the application of aptamers for the detection of infective agents itself, antigens/toxins (bacteria), biomarkers (cancer), or a combination. The ease of conjugation and labelling of aptamers makesthem a potential tool for diagnostics. Also, due to the reduced off-target effects of aptamers, their use as a potentialdrug delivery tool is emerging rapidly. Hence, we discuss their use in targeted delivery in conjugation with siRNAs,nanoparticles, liposomes, drugs and antibodies. Finally, we discuss about the conjugation strategies applicable forRNA and DNA aptamers for imaging. Their stability and self-assembly after heating makes them superior overprotein-based binding molecules in terms of labelling and conjugation strategies.

【 授权许可】

Unknown