Herpes simplex virus types 1 and 2 modulate autophagy in SIRC corneal cells

Journal of biosciences

Kata Pásztor¹  Goran Petrovski³  Klára Megyeri3¹  Kai Kaarniranta⁴  Réka Albert³  Edina Mencel¹  László Orosz¹  Morten C Moe⁵  Andrea Facskó² 

[1] Department of Medical Microbiology and Immunobiology, University of Szeged, Szeged, Hungary$$;Department of Ophthalmology, University of Szeged, Szeged, Hungary$$;Department of Ophthalmology, Medical and Health Science Center, University of Debrecen, Debrecen, Hungary$$;Department of Ophthalmology, Institute of Clinical Medicine, University of Eastern Finland, and Department of Ophthalmology, Kuopio University Hospital, Kuopio, Finland$$;Department of Ophthalmology, Center of Eye Research, Oslo University Hospital, University of Oslo, Oslo, Norway$$

关键词: Apoptosis;    autophagy;    corneal cells;    Herpes simplex virus;    keratitis;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Autophagy and apoptosis function as important early cellular defense mechanisms in infections and other diseases. The outcome of an infection is determined by a complex interplay between the pathogenic microorganism and these intracellular pathways. To better understand the cytopathogenicity of Herpes simplex virus types 1 and 2 (HSV-1 and - 2), we studied the effect of these viruses on the autophagic and apoptotic processes in the SIRC corneal cell line. Infection with the KOS strain of HSV-1 and a wild-type strain of HSV-2 enhanced autophagosome formation, triggered cytoplasmic acidification, increased LC3B lipidation and elevated the ratio of apoptotic cells. The autophagy inhibitor bafilomycin A1 triggered a significant increase in the apoptotic responses of HSV-1- and HSV-2-infected cells. Thus, both HSV types affect autophagy and apoptosis in a coordinated fashion, and autophagy plays cytoprotective role in HSV-infected cells via antagonizing apoptosis. Together these data implicate autophagy in the pathogenic mechanism of herpetic keratitis.

【 授权许可】

Unknown