Application of speckle image correlation for real-time assessment of metabolic activity in herpes virus-infected cells

1st International Scientific School on Methods of Digital Image Processing in Optics and Photonics

物理学;计算机科学

Vladimirov, A.P.^1,2 ; Malygin, A.S.^1 ; Mikhailova, J.A.^1,2 ; Borodin, E.M.^1 ; Bakharev, A.A.^2 ; Poryvayeva, A.P.^2

Ural Federal University, Mira street 19, Yekaterinburg

620002, Russia^1

FSSI Yekaterinburg Research Institute of Viral Infections of Rospotrebnadzor, Letnaya Street 23, Yekaterinburg

620030, Russia^2

关键词: Development process;    Glass substrates;    Herpes simplex virus;    Metabolic activity;    Real-time assessment;    Speckle dynamics;    Speckle interferometry;    Virus-infected cells;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/536/1/012012/pdf
DOI  :  10.1088/1742-6596/536/1/012012

学科分类：计算机科学（综合）

来源: IOP

【 摘 要 】

Earlier we reported developing a speckle interferometry technique and a device designed to assess the metabolic activity of a cell monolayer cultivated on a glass substrate. This paper aimed at upgrading the technique and studying its potential for real-time assessment of herpes virus development process. Speckle dynamics was recorded in the image plane of intact and virus-infected cell monolayer. HLE-3, L-41 and Vero cells were chosen as research targets. Herpes simplex virus-1-(HSV-1)- infected cell cultures were studied. For 24 h we recorded the digital value of optical signal I in one pixel and parameter η characterizing change in the distribution of the optical signal on 10 × 10-pixel areas. The coefficient of multiple determination calculated by η time dependences for three intact cell cultures equals 0.94. It was demonstrated that the activity parameters are significantly different for intact and virus-infected cells. The difference of η value for intact and HSV-1-infected cells is detectable 10 minutes from the experiment start.