Analysis of Rheb in the cellular slime mold Dictyostelium discoideum: Cellular localization, spatial expression and overexpression

Journal of biosciences

Shweta Saran1¹  Pooja Bhadoriya¹  Pynskhem Bok Swer¹ 

[1] School of Life Science, Jawaharlal Nehru University, New Delhi 110 067, India$$

关键词: Dictyostelium;    Rheb;    TOR signalling;   

DOI  :  

来源: Indian Academy of Sciences

【 摘 要 】

Dictyostelium discoideum encodes a single Rheb protein showing sequence similarity to human homologues of Rheb. The DdRheb protein shares 52% identity and 100% similarity with the human Rheb1 protein. Fluorescence of Rheb yellow fluorescent protein fusion was detected in the D. discoideum cytoplasm. Reverse transcription-polymerase chain reaction and whole-mount in situ hybridization analyses showed that rheb is expressed at all stages of development and in prestalk cells in the multicellular structures developed. When the expression of rheb as a fusion with lacZ was driven under its own promoter, the 𝛽-galactosidase activity was seen in the prestalk cells. D. discoideum overexpressing Rheb shows an increase in the size of the cell. Treatment of the overexpressing Rheb cells with rapamycin confirms its involvement in the TOR signalling pathway.

【 授权许可】

Unknown