New RP-HPLC Method for The Determination of Olmesartan Medoxomil in Tablet Dosage Form

Eurasian Journal of Analytical Chemistry

Ramprasad A. Lanka¹  Jayachandra R. Peddareddigari¹  Srinivasu Pamidi¹  Mustafa Mohammed¹  Raveendra Babu Ganduri¹ 

关键词: High performance thin layer chromatography;    densitometry;    validation;    quantification;    domperidone maleate;    naproxen sodium;   

DOI  :  10.12973/ejac.2010.00091a

学科分类：分析化学

来源: Eurasian Journal of Analytical Chemistry

【 摘 要 】

A simple rapid, sensitive, accurate, precise and reproducible high performance liquid chromatographic method has been developed to assay Olmesartan medoxomil in tablet dosage form. The HPLC analysis used a reversed phase Hypersil BDS C8 (250X4.6mm, 5µm) column and a mobile phase constituted of buffer and acetonitrile (55:45 % v/v). The buffer is composed of 3gm of sodium perchlorate and 3ml of tri ethyl amine in 1000 mL of water and the pH of the solution was adjusted to 3.0 with orthophosphoric acid. The wave length of the detection is 250 nm. The validation data showed that the assay is sensitive, specific and reproducible for the determination of olmesartan in the dosage form. The method is linear from 10µg mL-1 to 120µg mL-1. The accuracy of the method was found to be 99.54%. Mean inter and intraday assay relative standard deviation (RSD) were less than 1.0%. The proposed method provided an accurate and precise analysis of olmesartan in its pharmaceutical dosage form.

【 授权许可】

Unknown