Phyllanthin biosynthesis in Phyllanthus amarus: Schum and Thonn growing at different altitudes

Dynamic Chiropractic

Fahad Al-Qurainy¹  Salim Khan²  Mauji Ram³ 

[1] Centre for Transgenic Plant Development, Department of Biotechnology Faculty of Science, Jamia Hamdard, Hamdard Nagar, New Delhi, 110062, India.;Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia.;Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, Hamdard Nagar, New Delhi, 110062, India.

关键词: High performance thin layer chromatography;    phyllanthin;    quantification;    secondary metabolite.;   

DOI  :  10.5897/JMPR09.369

学科分类：医学（综合）

来源: MPA Media

【 摘 要 】

Phyllanthus amarusSchum and Thonn is a member offamilyEuphorbiaceaeand highly used in Indian system of medicine to cure numerous human ailments. The lignans, Phyllanthin and hypophyllanthin present in plant are reported as therapeutically active constituents and serve as hepatoprotective agents. We studied on 23 natural populations ofP. amarus, collected from different geographical regions of India, which were differed in altitude (114 - 5295 ft). The phyllanthin content was extracted and quantified in different parts ofP. amarususing high performance thin layer chromatography (HPTLC) method at 282 nm wavelength. Out of 23 populations, 16 populations showed increased phyllanthin content with elevated altitude. The highest amount of phyllanthin was found in the leaves followed by fruits and stem, whilst the roots have the least amount of phyllanthin. The highest and lowest phyllanthin content on dry weight basis was 3.163 mg/g in population P21at 2214 ft and 1.431 mg/g in population P2at 114 ft and a positive correlation was observed in 16 populations for phyllanthin content with the increased altitude. These results showed the importance of habitat characteristics in the biosynthesis of phyllanthin compound and possible mechanism may involve their antioxidant activity.

【 授权许可】

Unknown