Chlamydophila psittaci subclinical infection in chronic polyarthritis.

Clinical and Experimental Rheumatology

Piercarlo Sarzi-Puttini¹  Pia Morassi¹  Luca Quartuccio¹  Raffaele Pellerito¹  Sandra Lombardi¹  Salvatore De Vita¹  Francesco Curcio¹  Elisa Pasini¹  Domenico Biasi¹  Riccardo Dolcetti¹  Maurizio Benucci¹  Cinzia Fabro¹  Elena Pontarini¹  Martina Fabris¹ 

关键词: Chlamydophila psittaci;    Autoimmunity;    Chlamydophila psittaci;    Rheumatoid arthritis;    Chlamydophila psittaci;    pathogenesis;    rheumatoid factor;    anti-citrullinated peptides antibodies;   

DOI  :  

学科分类：医学（综合）

来源: Pacini Editore SpA

【 摘 要 】

OBJECTIVES: Recent evidence indicates that Chlamydophila psittaci (Cp) may establish chronic infections, which may promote autoimmunity and/or B cell lymphoproliferation. METHODS: The presence of a subclinical Cp infection was investigated in 293 patients with chronic inflammatory polyarthritis, including 175 patients with rheumatoid factor (RF)-positive and/or anti-CCP-positive rheumatoid arthritis (RA) and 118 with seronegative polyarthritis (46 RF-negative/anti-CCP-negative RA, 36 psoriatic arthritis and 36 undifferentiated spondyloarthritis). One hundred and eighty-five healthy controls were also investigated. The presence of Cp infection was assessed in peripheral blood mononuclear cells using several PCR protocols targeting different regions of the Cp genome (16S-23S spacer rRNA, OMP-A, and Gro-EL). The DNA of other Chlamydia species (C. Pneumoniae and C. Trachomatis) was also investigated. Amplicons were sequenced to confirm the specificity of PCR products. RESULTS: The presence of a subclinical chronic Cp infection was observed in a significantly higher percentage of patients with chronic polyarthritis (38/293; 13%) compared to healthy controls (1/185, 0.5%; OR=27.4, 95%CI:3.73-201.6, p

【 授权许可】

Unknown