Detection of Chlamydophila psittaci by Using SYBR Green Real-Time PCR

Journal of Veterinary Medical Science

Hideko OKUDA¹  Kenji OHYA¹  Hideto FUKUSHI¹  Yukihiro SHIOTA¹  Hiroshi KATO¹ 

[1] Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University

关键词: avian chlamydiosis;    Chlamydophila psittaci;    real-time PCR;    SYBR Green dye;   

DOI  :  10.1292/jvms.10-0222

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(2)Chlamydophila psittaci is the causative agent of human psittacosis and avian chlamydiosis. This zoonotic pathogen is frequently transmitted from infected birds to humans. Therefore proper and rapid detection of C. psittaci in birds is important to control this disease. We developed a method for detecting C. psittaci by using SYBR Green Real-time PCR based on targeting the cysteine-rich protein gene (envB) of C. psittaci. This one step procedure was highly sensitive and rapid for detection and quantification of C. psittaci from fecal samples. This assay was also able to detect other zoonotic Chlamydophila species such as C. abortus and C. felis. The assay is well suited for use as a routine detection method in veterinary medicine.

【 授权许可】

Unknown