FAD assembly and thylakoid membrane binding of ferredoxin:NADP+ oxidoreductase in chloroplasts

FEBS Letters

Hase, Toshiharu¹  Onda, Yayoi¹ 

[1] Division of Enzymology, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan

关键词: Ferredoxin:NADP+ oxidoreductase;    Ferredoxin;    Flavin adenine dinucleotide assembly;    Thylakoid membrane binding;    Chloroplast import;    Fd;    ferredoxin;    FNR;    ferredoxin:NADP+ oxidoreductase;    pFNR;    precursor FNR;    mFNR;    mature-sized FNR;   

DOI  :  10.1016/S0014-5793(04)00325-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the process of flavin adenine dinucleotide (FAD) incorporation into the ferredoxin (Fd):NADP⁺ oxidoreductase (FNR) polypeptide during FNR biosynthesis, using pull-down assay with resin-immobilized Fd which bound strongly to FAD-assembled holo-FNR, but hardly to FAD-deficient apo-FNR. After FNR precursor was imported into isolated chloroplasts and processed to the mature size, the molecular form pulled down by Fd-resin increasingly appeared. The mature-sized FNR (mFNR) accumulated transiently in the stroma as the apo-form, and subsequently bound on the thylakoid membranes as the holo-form. Thus, FAD is incorporated into the mFNR inside chloroplasts, and this assembly process is followed by the thylakoid membrane localization of FNR.

【 授权许可】

Unknown