The PSI‐E subunit of photosystem I binds ferredoxin:NADP+ oxidoreductase

FEBS Letters

Møller, Birger Lindberg¹  Andersen, Birgitte¹  Scheller, Henrik Vibe¹ 

[1] Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, 40 Trorvaldsensvej, DK-1871 Frederiksberg C, Copenhagen, Denmark

关键词: Photosystem I;    Ferredoxin:NADP+ oxidoreductase;    Cross-linking;    PSI-E;    FNR-binding protein;    chl;    chlorophyll;    DM;    n-decyl-β-d-maltopyranoside;    DM-PS I;    PS I purified with n-decyl-β-d-maltopyranoside;    DTSSP;    3;    3′-dithiobis(sulfosuccinimidylpropionate);    EDC;    N-ethyl-3-[3-(dimethylamino)propyl]carbodiimide;    FNR;    ferredoxin:NADP+oxidoreductase;    LHC I;    light-harvesting complex I;    MES;    4-morpholine-ethanesulphonic acid;    PS I;    photosystem I;    SDS;    sodium dodecyl sulfate;    Tricine;    N-[2-hydroxy-1;    I-bis(hydromethyl)-ethyl]glycine;    TX-PS I;    PS I purified with Triton X-100;   

DOI  :  10.1016/0014-5793(92)81391-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A photosystem I complex containing the polypeptides PSI-A to PSI-L, light-harvesting complex I and ferredoxin:NADP⁺ oxidoreductase has been isolated from barley using the non-ionic detergent n-decyl-β-d-maltopyranoside. The ratio between bound forredoxin:NADP⁺ oxidoreductase and P700 is 0.4 ± 0.2. The complex is highly active in catalyzing light-induced transfer of electrons from plastocyanin to NADP⁺ at rates of 280±150 and 1800 ± 800, μmol NADPH/(mg chl h), without and in the presence of saturating amounts of exogenously added ferredoxin:NADP₊ oxidoreductase, respectively. Endogenously bound ferredoxin:NADP⁺ oxidoreductase interacts with the PSI-E subunit as demonstrated by cross-linking experiments using two different types of cross-linkers and identification of the products by Western blotting and the use of monospecific antibodies.

【 授权许可】

Unknown