Selective interaction of ferricyanide with cluster I of Clostridium pasteurianum 2[Fe4S4] ferredoxin

FEBS Letters

Messori, Luigi¹  Bertini, Ivano¹  Briganti, Fabrizio¹  Calzolai, Luigi¹  Scozzafava, Andrea¹ 

[1] Department of Chemistry, University of Florence, via Gino Capponi 7, I-50121 Florence, Italy

关键词: Ferredoxin;    NMR;    Clostridium pasteurianum;   

DOI  :  10.1016/0014-5793(93)80647-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of Clostridium pasteurianum ferredoxin (CpFd) with stoichiometric amounts of potassium ferricyanide results in the specific conversion of cluster I into a Fe₃S₄ ⁺ species while leaving cluster II unaltered. Ferricyanide-treated CpFd derivative has been purified and characterized through biochemical and spectroscopical techniques. The cluster conversion process is reversible and reconstitution of native CpFd has been afforded under appropriate conditions.

【 授权许可】

Unknown