The inhibition of cell spreading on a cellulose substrate (cuprophan) induces an apoptotic process via a mitochondria‐dependent pathway

FEBS Letters

Gekas, Jean²  Fuentès, Vincent¹  Nagel, Marie-Danielle²  Gouilleux-Gruart, Valérie¹  Lanvin, Olivia¹  Mazière, Cécile¹  David, Bertrand²  Mazière, Jean-Claude¹  Hindié, Mathilde²  Lassoued, Kaı̈ss¹  Faucheux, Nathalie² 

[1] Laboratoire d’immunologie INSERM E0351, Faculté de Médecine, 3, rue des Louvels, 80036 Amiens, France;Domaine Biomatériaux-Biocompatibilité, UMR CNRS 6600, Université de Technologie de Compiègne, P.O. Box 20529, F-60205 Compiègne Cedex, France

关键词: Apoptosis;    Caspase;    Cell aggregation;    Biomaterial;    Fibroblast;   

DOI  :  10.1016/S0014-5793(04)00276-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cell shape was found to be a strong indicator of whether individual cells grow or die, and may play an important role in controlling apoptosis as well as cell growth. We compared here the behaviour of rounded Swiss 3T3 cells aggregated on a cellulose cuprophan membrane to those cultured on dish polystyrene. We demonstrated that cells aggregated on cellulose substrates for up to 48 h underwent programmed cell death that was associated with phosphatidylserine flipping and caspase 9 and caspase 3 activation, suggesting a mitochondria-dependent apoptotic process. In addition, we found that this phenomenon cannot be entirely explained by disengagement of α5β1 integrin ligation.

【 授权许可】

Unknown