Glucosylceramides in Colletotrichum gloeosporioides are involved in the differentiation of conidia into mycelial cells

FEBS Letters

Rodrigues, Marcio L²  Almeida, Igor C¹  Barreto-Bergter, Eliana²  Farias, Sandra E³  da Silva, André F.C²  Pinto, Márcia R² 

[1] Departamento de Parasitologia, Universidade de São Paulo, São Paulo, Brazil;Instituto de Microbiologia Professor Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco I, Cidade Universitária, Rio de Janeiro 21941-590, Brazil;Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil

关键词: Glucosylceramide;    Monoclonal antibody to GlcCer;    Colletotrichum gloeosporioides;    GlcCer;    glucosylceramides;    ESI-MS;    electrospray ionization mass spectrometry;    NMR;    nuclear magnetic resonance;    GC;    gas chromatography;   

DOI  :  10.1016/S0014-5793(04)00156-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glucosylceramides (GlcCer) were extracted from the plant pathogen Colletotrichum gloeosporioides and purified by several chromatographic steps. By using electrospray ionization mass spectrometry and nuclear magnetic resonance, GlcCer from C. gloeosporioides were identified as N-2′-hydroxyoctadecanoyl-1-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine and N-2′-hydroxyoctadecenoyl-1-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine. Monoclonal antibodies against these structures were produced and used as tools for the evaluation of the role of GlcCer in the morphological transition of C. gloeosporioides. In the presence of antibodies to GlcCer, the differentiation of conidia into mycelia was blocked. Since GlcCer is present in several plant pathogens, the inhibitory activity of external ligands recognizing these structures may be applicable in other models of fungal infections.

【 授权许可】

Unknown