Extensive precursor‐product relationship between gangliosides formed from exogenous glucosylceramide in rat liver

FEBS Letters

Vallenzasca, Carlo¹  Trinchera, Marco¹  Ghidoni, Riccardo¹  Tettamanti, Guido¹ 

[1] Study Center for the Functional Biochemistry of Brain Lipids, Department of Medical Chemistry and Biochemistry, University of Milan, Italy

关键词: Glucosylceramide;    Ganglioside;    Golgi apparatus;    Glycosylation;   

DOI  :  10.1016/0014-5793(90)80056-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glucosylceramide, radiolabelled on the glucose residue, was administered to rats and the radioactive gangliosides formed at different time points were chemically characterized. They were identified as GM3, GM1, GD1a and GD1b, each one carrying only radioactive glucose. The time course of each individual ganglioside showed that the simpler gangliosides were formed earlier but were consumed earlier than the more complex ones, resulting in radioactivity patterns that were different at each time point. Only 30 h after injection did it resemble that of endogenous rat liver gangliosides. These results indicate that an extensive precursor-product relationship actually exists in the course of ganglioside biosynthesis.

【 授权许可】

Unknown