FEBS Letters | |
The artificial zinc finger protein ‘Blues’ binds the enhancer of the fibroblast growth factor 4 and represses transcription | |
Passananti, C2  Corbi, N2  Onori, A2  Fanciulli, M1  Libri, V2  | |
[1] Laboratorio ‘B’, Regina Elena Cancer Institute, Via delle Messi d’Oro 156, 00158 Rome, Italy;Istituto Biologia e Patologia Molecolari, CNR, Viale Marx 43, 00137 Rome, Italy | |
关键词: Artificial transcription factor; Zinc finger; Recognition code; Fibroblast growth factor 4; Oncogene; | |
DOI : 10.1016/S0014-5793(04)00075-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The design of novel genes encoding artificial transcription factors represents a powerful tool in biotechnology and medicine. We have engineered a new zinc finger-based transcription factor, named Blues, able to bind and possibly to modify the expression of fibroblast growth factor 4 (FGF-4, K-fgf), originally identified as an oncogene. Blues encodes a three zinc finger peptide and was constructed to target the 9 bp DNA sequence: 5′-GTT-TGG-ATG-3′, internal to the murine FGF-4 enhancer, in proximity of Sox-2 and Oct-3 DNA binding sites. Our final aim is to generate a model system based on artificial zinc finger genes to study the biological role of FGF-4 during development and tumorigenesis.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020313881ZK.pdf | 237KB | download |