The artificial zinc finger protein ‘Blues’ binds the enhancer of the fibroblast growth factor 4 and represses transcription

FEBS Letters

Passananti, C²  Corbi, N²  Onori, A²  Fanciulli, M¹  Libri, V² 

[1] Laboratorio ‘B’, Regina Elena Cancer Institute, Via delle Messi d’Oro 156, 00158 Rome, Italy;Istituto Biologia e Patologia Molecolari, CNR, Viale Marx 43, 00137 Rome, Italy

关键词: Artificial transcription factor;    Zinc finger;    Recognition code;    Fibroblast growth factor 4;    Oncogene;   

DOI  :  10.1016/S0014-5793(04)00075-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The design of novel genes encoding artificial transcription factors represents a powerful tool in biotechnology and medicine. We have engineered a new zinc finger-based transcription factor, named Blues, able to bind and possibly to modify the expression of fibroblast growth factor 4 (FGF-4, K-fgf), originally identified as an oncogene. Blues encodes a three zinc finger peptide and was constructed to target the 9 bp DNA sequence: 5′-GTT-TGG-ATG-3′, internal to the murine FGF-4 enhancer, in proximity of Sox-2 and Oct-3 DNA binding sites. Our final aim is to generate a model system based on artificial zinc finger genes to study the biological role of FGF-4 during development and tumorigenesis.

【 授权许可】

Unknown