FEBS Letters | |
Synthesis of a new zinc finger peptide; comparison of its `code' deduced and `CASTing' derived binding sites | |
Passananti, Claudio1  Maione, Rossella2  Perez, Marie2  Corbi, Nicoletta2  | |
[1] Istituto di Tecnologie Biomediche, CNR, Via Morgagni 30/E, 00161 Rome, Italy;Dipartimento di Biotecnologie Cellulari ed Ematologia, Università di Roma La Sapienza, Viale Regina Elena 324, 00161 Rome, Italy | |
关键词: Zinc finger-DNA interaction; Protein design; Recognition code; Casting selection; Dissociation constant; | |
DOI : 10.1016/S0014-5793(97)01257-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Using two synthetic oligonucleotides, we have constructed a new gene containing three zinc finger motifs of the Cys2-His2 type. We named this artificial gene `Mago'. The Mago nucleotide triplets encoding the amino acid positions, described to be crucial for DNA binding specificity, have been chosen on the basis of the proposed recognition `code' that relates the zinc finger's primary structure to the DNA binding target. Here we demonstrate that Mago protein specifically binds the `code' DNA target, with a dissociation constant (K d) comparable to the K d of the well known Zif268 protein with its binding site. Moreover, we show that the deduced Mago `code' and the `experimental' selected DNA binding sites are almost identical, differing only in two nucleotides at the side positions.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020305142ZK.pdf | 595KB | download |