Histidine‐containing phosphotransfer domain extinction by RNA interference turns off a cytokinin signalling circuitry in Catharanthus roseus suspension cells

FEBS Letters

Vansiri, Amerin¹  Papon, Nicolas¹  Crèche, Joël¹  Rideau, Marc²  Gantet, Pascal¹  Chénieux, Jean-Claude² 

[1] EA 2106, Plant Molecular Biology and Biochemistry Department, Faculty of Pharmacy, 31 avenue Monge, F-37200 Tours, France;Unité sous Contrat reconnue par l'INRA ‘Facteurs de transcription et ingénierie métabolique végétale’, Université de Tours, Tours, France

关键词: Histidine-containing phosphotransfer;    Cytokinin;    Catharanthus roseus;   

DOI  :  10.1016/S0014-5793(03)01522-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously reported that cytokinins (CK) induce the fast and specific transcription of CrRR1, a gene encoding a type A response regulator in Catharanthus roseus cell cultures. Here, we characterized the CrHPt1 gene that encodes a histidine-containing phosphotransfer domain. CrHPt1 was silenced through RNA interference (RNAi) to test its possible implication in the CK signalling pathway. In transgenic lines stably transformed with an intron-spliced construct, the degradation of CrHPt1 transcripts abolishes the CK inductive effect on CrRR1 transcription. These result give a new in vivo functional argument for the crucial role of HPt proteins in the CK signalling pathway leading to the expression of the genes encoding type A response regulators. They also show that RNAi is a powerful strategy to turn off the CK signalling circuitry.

【 授权许可】

Unknown