Conjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cells

FEBS Letters

Eccles, Michael R²  Muratovska, Aleksandra¹ 

[1] MRC-Dunn Human Nutrition Unit, Wellcome Trust/MRC building, Hills Rd, Cambridge CB2 2XY, UK;Developmental Genetics Laboratory, Department of Pathology, Dunedin School of Medicine, University of Otago, P.O. Box 913, Dunedin, New Zealand

关键词: Short interfering RNA;    Penetratin;    Transportan;    Transfection;    Gene silencing;    MPP;    membrane permeant peptide;    siRNA;    short interfering RNA;    GFP;    green fluorescent protein;    dsRNA;    double-stranded RNA;   

DOI  :  10.1016/S0014-5793(03)01505-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The efficient delivery of short interfering RNAs (siRNAs) into cells provides a powerful approach to study cellular functions. SiRNAs were coupled to the membrane permeant peptides (MPPs) penetratin and transportan to improve their uptake by cells. Thiol-containing siRNAs corresponding to luciferase, or green fluorescent protein (GFP) transgenes, were synthesized and conjugated to penetratin or transportan via a disulfide bond that is labile in the reducing environment of the cytoplasm. These MPP-siRNAs efficiently reduced transient and stable expression of reporter transgenes in several mammalian cell types in a high proportion of cells, and demonstrated equivalent or better delivery characteristics than cationic liposomes with fewer manipulations.

【 授权许可】

Unknown