The Antennapedia peptide penetratin translocates across lipid bilayers – the first direct observation

FEBS Letters

Nordén, Bengt²  Karlsson, Mattias¹  Persson, Daniel²  Thorén, Per E.G.² 

[1] Department of Chemistry, Göteborg University, SE-412 96 Gothenburg, Sweden;Department of Physical Chemistry, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden

关键词: Antennapedia homeodomain;    Penetratin;    Membrane translocation;    Delivery vector;    Uptake;   

DOI  :  10.1016/S0014-5793(00)02072-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The potential use of polypeptides and oligonucleotides for therapeutical purposes has been questioned because of their inherently poor cellular uptake. However, the 16-mer oligopeptide penetratin, derived from the homeodomain of Antennapedia, has been reported to enter cells readily via a non-endocytotic and receptor- and transporter-independent pathway, even when conjugated to large hydrophilic molecules. We here present the first study where penetratin is shown to traverse a pure lipid bilayer. The results support the idea that the uptake mechanism involves only the interaction of the peptide with the membrane lipids. Furthermore, we conclude that the translocation does not involve pore formation.

【 授权许可】

Unknown