Penetratin‐induced aggregation and subsequent dissociation of negatively charged phospholipid vesicles

FEBS Letters

Nordén, Bengt¹  Thorén, Per E.G¹  Persson, Daniel¹ 

[1] Department of Physical Chemistry, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden

关键词: Antennapedia homeodomain;    Penetratin;    Vesicle aggregation;    Circular dichroism;    Artifact;    Turbidity;    CD;    circular dichroism;    DOPG;    1;    2-dioleoyl-sn-glycero-3-phosphoglycerol;    DMPG;    1;    2-dimyristoyl-sn-glycero-3-phosphoglycerol;    EDTA;    ethylenediaminetetraacetic acid;    HEPES;    N-(2-hydroxyethyl)-piperazine-N′-(2-ethanesulfonic acid);    LUV;    large unilamellar vesicle;    DLS;    dynamic light scattering;   

DOI  :  10.1016/S0014-5793(01)02843-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction of the cellular delivery vector penetratin with a model system consisting of negatively charged phospholipid vesicles has been studied. Above a certain peptide to lipid molar ratio, the cationic oligopeptide induces vesicle aggregation. Interestingly, the aggregation is followed by spontaneous disaggregation, which may be related to membrane translocation of the peptide. Circular dichroism (CD) measurements indicate a conformational transition, from α-helix to antiparallel β-pleated sheet, which is simultaneous with the aggregation process. The potential influence of spectroscopic artifacts on CD data due to the drastically increased turbidity during aggregation is discussed.

【 授权许可】

Unknown