A plant outer mitochondrial membrane protein with high amino acid sequence identity to a chloroplast protein import receptor

FEBS Letters

Millar, A.Harvey²  Chew, Orinda²  Qbadou, Soumya¹  Lister, Ryan²  Schleiff, Enrico¹  Soll, Jurgen¹  Heazlewood, Joshua L²  Whelan, James² 

[1] LMU München, Menzinger Strasse 67, 80638 Munich, Germany;Plant Molecular Biology Group, School of Biomedical and Chemical Sciences, The University of Western Australia, 35 Stirling Highway, Nedlands, WA 6009, Australia

关键词: Mitochondrion;    Chloroplast;    Receptor;    Import;    Expression;    Proteome;    TOM;    translocase of the outer membrane of mitochondria;    TIM;    translocase of the inner membrane of mitochondria;    Toc;    translocase of the outer envelope membrane of chloroplasts;    TPR;    tetratricopeptide repeat;   

DOI  :  10.1016/S0014-5793(03)01457-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified a novel protein on the outer membrane of Arabidopsis thaliana mitochondria. This protein displays 67% sequence identity with the 64 kDa translocase of the outer envelope membrane of chloroplasts (Toc). A mitochondrial localisation for this protein was determined by (i) its presence in the proteome of highly purified Arabidopsis mitochondria, (ii) Western blot analysis with antibodies to Toc64 from pea that indicate its presence in Arabidopsis and pea mitochondria, (iii) green fluorescent protein fusion proteins that indicate an exclusive mitochondrial localisation for this protein, and (iv) expression profiles in various tissue types and during development that are more similar to translocase of the outer mitochondrial membrane components than to chloroplastic Toc components. Thus Arabidopsis mitochondria contain a protein with high sequence identity to a plastid protein import receptor.

【 授权许可】

Unknown