The outer envelope protein OEP24 from pea chloroplasts can functionally replace the mitochondrial VDAC in yeast

FEBS Letters

Motzkus, Michael¹  Soll, Jürgen¹  Röhl, Thomas¹ 

[1] Botanisches Institut, Universität Kiel, Am Botanischen Garten 1-9, D-24118 Kiel, Germany

关键词: Chloroplast;    Mitochondrion;    Yeast complementation;    Outer envelope protein;    Pisum sativum;    Saccharomyces cerevisiae;    OEP;    outer envelope protein;    VDAC;    voltage-dependent anion channel;    TOM;    translocon outer mitochondrial membrane;    TIM;    translocon inner mitochondrial membrane;   

DOI  :  10.1016/S0014-5793(99)01399-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The chloroplastic outer envelope protein OEP24 from pea forms a high-conductance low specificity solute channel as shown by in vitro studies. In order to establish its function also in an in vivo-like system, the gene encoding OEP24 was transformed into a yeast strain which lacks the general mitochondria solute channel porin, also known as voltage-dependent anion channel (VDAC). Transformation of the yeast VDAC(−) strain with the OEP24 gene resulted in the recovery of a phenotype indistinguishable from the wild-type. The OEP24 polypeptide is targeted to the mitochondrial outer membrane in this heterologous system. We conclude that OEP24 forms a solute channel in pea chloroplasts in planta.

【 授权许可】

Unknown