Lipid raft‐associated catechin suppresses the FcϵRI expression by inhibiting phosphorylation of the extracellular signal‐regulated kinase1/2

FEBS Letters

Fujimura, Yoshinori¹  Tachibana, Hirofumi¹  Yamada, Koji¹ 

[1]Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan

关键词: High-affinity IgE receptor;    (−)-Epigallocatechin-3-O-gallate;    Tea catechin;    Lipid raft;    Extracellular signal-regulated kinase;    KU812;    CTXB;    cholera toxin B;    EGCG;    (−)-epigallocatechin-3-O-gallate;    ERK1/2;    extracellular signal-regulated kinase1/2;    FcϵRI;    high-affinity IgE receptor;    HPLC;    high-performance liquid chromatography;    G3PDH;    glyceraldehyde-3-phosphate dehydrogenase;    JNK;    c-jun N-terminal kinase;    MAPK;    mitogen-activated protein kinase;    MβCD;    methyl-β-cyclodextrin;    RT-PCR;    reverse transcription-polymerase chain reaction;    SPR;    surface plasmon resonance;   

DOI  :  10.1016/S0014-5793(03)01432-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The major green tea catechin, (−)-epigallocatechin-3-O-gallate (EGCG), has a suppressive effect on the expression of the high-affinity IgE receptor FcϵRI, which is key molecule in the IgE-mediated allergic reactions. Here we show that EGCG binds to the cell surface and highly associates with plasma membrane microdomains, lipid rafts, on the human basophilic KU812 cells. The disruption of these lipid rafts caused a reduction of the amount of raft-associated EGCG and the FcϵRI-suppressive effect of EGCG. We also found that EGCG has an ability to inhibit the phosphorylation of the extracellular signal-regulated kinase1/2 (ERK1/2) and that the ERK1/2 specific inhibitor also reduced FcϵRI expression. Moreover, the inhibitory effect elicited by EGCG on ERK1/2 was prevented by disruption of rafts. Thus, these results suggest that the interaction between EGCG and the lipid rafts is important for EGCG's ability to downregulate FcϵRI expression, and ERK1/2 may be involved in this suppression signal.

【 授权许可】

Unknown