Determination of the sequence coding for the β subunit of the human high‐affinity IgE receptor

FEBS Letters

Tanaka, Yoshiaki²  Harada, Shigenori¹  Imagawa, Noriko¹  Maekawa, Kazuhiko¹ 

[1] Shionogi Institute for Medical Science, 2-5-1, Mishima, Settsu-shi, Osaka 566, Japan;Department of Obstetrics and Gynecology, Suita Municipal Hospital, Suita, Osaka 564, Japan

关键词: High-affinity IgE receptor;    Human β subunit;    Cloning;    Sequencing;    Polymerase chain reaction;    Polymerase chain reaction walking;   

DOI  :  10.1016/0014-5793(92)80430-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cDNA encoding the β subunit of the human high-affinity IgE receptor was cloned by a combination or various polymerase chain reactions (PCR). A major portion of the β cDNA was amplified using primers homologous within the sequences of rat and mouse. The 3′ unknown sequence was preferentially amplified using the RNA template-specific PCR and the improved two-step PCR. The 5′ unknown sequence was specifically amplified by our newly developed PCR walking. Random heptanucleotides tagged with a unique sequence at the 5′ end were used as the walking primer. Finally, the entire coding region was amplified and sequenced. The two extracellular loops of the human β subunit were the least homologous to those of rat and mouse.

【 授权许可】

Unknown