AtPNP‐A is a systemically mobile natriuretic peptide immunoanalogue with a role in Arabidopsis thaliana cell volume regulation

FEBS Letters

Morse, Monique¹  Pironcheva, Ganka¹  Gehring, Chris¹ 

[1] University of the Western Cape, Department of Biotechnology, Private Bag X17, Bellville 7535, South Africa

关键词: Plant homeostasis;    Cell volume regulation;    Natriuretic peptide;    Plant protoplast;    Arabidopsis thaliana;    ANP;    atrial natriuretic peptide;    PNP;    plant natriuretic peptide;   

DOI  :  10.1016/S0014-5793(03)01384-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cellular and physiological evidence suggests the presence of a novel class of systemically mobile plant molecules that are recognized by antibodies against vertebrate atrial natriuretic peptides (ANPs). In order to characterize the function of these immunoanalogues we have expressed the full-length recombinant (AtPNP-A[1–126]) and demonstrate that this molecule induces osmoticum-dependent H₂O uptake into protoplasts at nanomolar concentrations and thus affects cell volume. A similar response is also seen with a recombinant that does not contain the signal peptide (AtPNP-A[26–126]) as well as a short domain (AtPNP-A[33–66]) that shows homology to the vertebrate peptide. Taken together, these findings suggest that AtPNP-A has an important and systemic role in plant growth and homeostasis.

【 授权许可】

Unknown