Gene transfer in plant protoplasts Inhibition of gene activity by cytosine methylation and expression of single‐stranded DNA constructs

FEBS Letters

Hershkovitz, Maty¹  Loyter, Abraham¹  Zakai, Nehama¹  Gruenbaum, Yosef² 

[1] Department of Biological Chemistry, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel;Department of Genetics, Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem 91904, Israel

关键词: Gene regulation;    Plant protoplast;    DNA methylation;   

DOI  :  10.1016/0014-5793(89)80952-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transfection of petunia protoplasts with the plasmid pUC118CaMVCAT, bearing the bacterial chloramphenicol acetyltransferase (CAT) gene, resulted in the appearance of CAT activity. No CAT activity was detected in protoplasts transfected with the hemimethylated form of pUC118CaMVCAT. Transfection of petunia protoplasts with ss DNA constructs bearing the coding and noncoding sequences of the CAT gene also resulted in the appearance of CAT activity, indicating expression of ss DNA constructs in transfected protoplasts. The relevance of our observations to control of gene expression in plant cells is discussed.

【 授权许可】

Unknown