Biological activity of hemimethylated and single‐stranded DNA after direct gene transfer into tobacco protoplasts

FEBS Letters

Graessmann, A.¹  Weber, H.¹ 

[1] Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-1000 Berlin 33, Germany

关键词: Hemimethylation;    Plant protoplast;    Gene expression;    DNA;    single-stranded;    DNA replication;   

DOI  :  10.1016/0014-5793(89)80951-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this investigation we analyzed the effect of DNA methylation on gene expression in tobacco protoplasts. Methylation of the chimeric GUS gene (CaMV 35s/GUS/NOS) with the HpaII methyltransferase had no effect on transient and on stable GUS gene expression. The chimeric gene was then cloned as single-stranded DNA. In vitro second-strand DNA synthesis was performed with 5-methylcytosine, leading to methylation of every cytosine in one DNA strand. After transfection this DNA was totally inactive. Control experiments revealed that the single-stranded DNA was as active as the double-stranded form.

【 授权许可】

Unknown