FEBS Letters | |
Thiamine‐regulated gene expression of Aspergillus oryzae thiA requires splicing of the intron containing a riboswitch‐like domain in the 5′‐UTR | |
Yamashita, Nobuo2  Kubodera, Takafumi2  Nishimura, Akira2  Yoshiuchi, Kumi2  Watanabe, Mutsumi2  Gomi, Katsuya1  Nakai, Susumu2  Hanamoto, Hideo2  | |
[1] Division of Bioscience and Biotechnology for Future Bioindustries, Graduate School of Agricultural Science, Tohoku University, 1-1, Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan;Research and Development Department, Hakutsuru Sake Brewing Co. Ltd., 4-5-5, Sumiyoshiminami-machi, Higashinada-ku, Kobe 658-0041, Japan | |
关键词: Thiamine; Gene regulation; Pyrithiamine; Intron splicing; Riboswitch; Aspergillus oryzae; 5′-UTR; 5′-untranslated region; GUS; β-glucuronidase; PT; pyrithiamine; TPP; thiamine pyrophosphate; | |
DOI : 10.1016/S0014-5793(03)01335-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Exogenous thiamine regulates Aspergillus oryzae thiA, which is involved in thiamine synthesis. One of the two introns in its 5′-untranslated region (5′-UTR) contains motifs (regions A and B) highly conserved among fungal thiamine biosynthesis genes. Deletion of either region relieved the repression by thiamine and thiamine inhibited intron splicing, suggesting that regions A and B are required for efficient splicing. Furthermore, transcript splicing was essential for thiA gene expression. These observations suggest a novel gene expression regulatory mechanism in filamentous fungi, in which exogenous thiamine controls intron splicing to regulate gene expression. Interestingly, regions A and B constitute a part of a thiamine pyrophosphate-binding riboswitch-like domain that has been quite recently found in the 5′-UTR of thiA.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020313673ZK.pdf | 1370KB | download |