Thiamine‐regulated gene expression of Aspergillus oryzae thiA requires splicing of the intron containing a riboswitch‐like domain in the 5′‐UTR

FEBS Letters

Yamashita, Nobuo²  Kubodera, Takafumi²  Nishimura, Akira²  Yoshiuchi, Kumi²  Watanabe, Mutsumi²  Gomi, Katsuya¹  Nakai, Susumu²  Hanamoto, Hideo² 

[1] Division of Bioscience and Biotechnology for Future Bioindustries, Graduate School of Agricultural Science, Tohoku University, 1-1, Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan;Research and Development Department, Hakutsuru Sake Brewing Co. Ltd., 4-5-5, Sumiyoshiminami-machi, Higashinada-ku, Kobe 658-0041, Japan

关键词: Thiamine;    Gene regulation;    Pyrithiamine;    Intron splicing;    Riboswitch;    Aspergillus oryzae;    5′-UTR;    5′-untranslated region;    GUS;    β-glucuronidase;    PT;    pyrithiamine;    TPP;    thiamine pyrophosphate;   

DOI  :  10.1016/S0014-5793(03)01335-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exogenous thiamine regulates Aspergillus oryzae thiA, which is involved in thiamine synthesis. One of the two introns in its 5′-untranslated region (5′-UTR) contains motifs (regions A and B) highly conserved among fungal thiamine biosynthesis genes. Deletion of either region relieved the repression by thiamine and thiamine inhibited intron splicing, suggesting that regions A and B are required for efficient splicing. Furthermore, transcript splicing was essential for thiA gene expression. These observations suggest a novel gene expression regulatory mechanism in filamentous fungi, in which exogenous thiamine controls intron splicing to regulate gene expression. Interestingly, regions A and B constitute a part of a thiamine pyrophosphate-binding riboswitch-like domain that has been quite recently found in the 5′-UTR of thiA.

【 授权许可】

Unknown